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Image Search Results
Journal: The Journal of Clinical Investigation
Article Title: The gut signals to AGRP-expressing cells of the pituitary to control glucose homeostasis
doi: 10.1172/JCI164185
Figure Lengend Snippet: ( A ) Cells expressing Agrp-B induced tdTomato fluorescence in the anterior pituitary of a female Agrp-B-FLP Ai193 mouse (original magnification, ×400). There is extensive tdTomato fluorescence in star-shaped cells that are distinctly separate from discrete, rounded CGA-ir hormone-expressing cells. An orange arrow in the merged image points to a double-labeled cell (AGRP-B + CGA + ). The pars nervosa has very weak, if any, tdTomato fluorescence. Scale bars: 50 μm. ( B and C ) Immunoreactivity for S100b ( B ) and SOX2 ( C ) in star-shaped cells of the anterior lobe that show extensive codistribution with the tdTomato label of a female Agrp-B-FLP Ai193 mouse. Scale bars: 100 μm. ( D ) Extensive labeling of FGFR1-ir with tdTomato-labeled star-shaped cells in the anterior lobe of a male Agrp-B-FLP Ai193 mouse. Scale bars: 50 μm. ( E ) A 5-day taurocholate (TC) treatment could significantly reduce AGRP-B mRNA in the pituitary of 2- to 3-month-old male mice ( P < 0.02, n = 6 per group). # P < 0.05, 1-tailed t test.
Article Snippet: We used the following primary antibodies: AGRP (guinea pig polyclonal, PA118414, Pierce Biotechnology), CGA (goat polyclonal, GtxHu-020-D, ImmunoReagents), DAGLB (rabbit polyclonal, NBP1-69662, Novus),
Techniques: Expressing, Fluorescence, Labeling